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朱雪萍 助理教授 (Hsueh-Ping Chu, Assistant Professor)


職 稱 助理教授
出生年 1978
最高學歷 美國羅格斯大學生物醫學 博士


專 長

非編碼 RNA 、表觀遺傳學

E-mail cchu2017@ntu.edu.tw


電話 02-33662487
傳真 02-33662478

1. 非編碼 RNA 與蛋白質和基因體間的交互作用

2. TERRA RNA 在染色體粒端的功能

由於定序技術 的 進步,科學界發現大於90%的基因體都會 表現 RNA ,其中 非編碼RNA占了絕大部分,非編碼RNA所指的是 不轉譯成蛋白 質的RNA, 其中 包含小片段的 microRNAs (miRNA) 和長片段 long noncoding RNAs (LncRNA s ) 。 科學界對 這些新發現的 非編碼 RNA 的功能還不甚了解。有文獻指出這些非編碼 RNA 對於發育與人類疾病都有很大的相關性,也發現他們會調控基因表現。本實驗室利用 CHIRT-seq 與 iDRiP-MS 的技術,以系統化的方法去研究非編碼 RNA 與蛋白質和基因體間的交互作用。藉由了解 非編碼 RNA 的功能,期望能找出新的方法治療人類疾病。

目前實驗室著力於研究 Telomeric-repeat containing RNA (TERRA) 的功能,它是一個 非編碼RNA,且帶有染色體粒端的 重 複 序列。文獻指出TERRA 跟癌症與疾病 ICF syndrome (immunodeficiency, centromere instability, and facial anomalies) 有密切相關性 . 我們之前的研究也發現TERRA 可調控基因表現與染色體粒端的維護。 染色體粒端的 作用是保持染色 體 的完整性,與 癌症和老化有關。

More than 90% of the genome is transcribed into non-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs). LncRNAs play important roles in regulating a wide range of cellular functions and developmental processes via function critically in the cis- and trans-regulation of gene expression, epigenetic modulation in the nucleus and post-transcriptional control in the cytoplasm. The dysregulation of lncRNAs has been linked to numerous human diseases. Our laboratory uses an RNA-centric approach to reveal the network of ncRNA-protein-genome. The long-term goal is to develop a therapeutic technology for human diseases by targeting ncRNAs.

Identify RNA-protein-DNA network by RNA-capture

TElomeric Repeat containing RNA (TERRA), synthesized at the telomeric ends and highly conserved in eukaryotic cells, is a rising star lncRNA due to its crucial association with the telomere maintenance and human genetic disorders including cancer and the ICF syndrome (immunodeficiency, centromere instability, and facial anomalies). Telomeres are specialized DNA-protein structures that protect the ends of linear chromosomes and maintain genomic integrity. Studies uncovered that while TERRA plays a major role in telomere dynamics, TERRA also binds to sites throughout the genome and regulates gene expression. We are interested in the mechanisms by which TERRA regulates the telomere function and gene expression.

Illustration of telomeric-repeat containing RNA (TERRA)

The oval image shows strand-specific staining for telomere using chromosome orientation fluorescence in situ hybridization (CO-FISH) followed by TERRA depletion. Overlapping of red and green signals indicates an event of telomere recombination.




1. Hsueh-Ping Chu , Catherine Cifuentes-Rojas , Barry Kesner, Eric Abey, Hyun Jung Oh, Myriam Boukhali, Wilhelm Hass, and Jeannie T. Lee. TERRA RNA Antagonizes ATRX and Protects Telomeres. Cell . 2017 June 29 Volume 170, p86-101.
[This paper was the subject of a perspective written by Roake CM, Artandi SE. Approaching TERRA Firma: Genomic Functions of Telomeric Noncoding RNA . Cell . 2017 Jun 29;170(1):8-9.]
[This paper was the subject of a perspective written by Koch L. Nat Rev Genet . 2017 Jul 17;18(8):453. Non-coding RNA : A protective role for TERRA at telomeres.

2.Hsueh-Ping Chu , Barry Kesner, John E. Froberg, Hyun Jung Oh, Stefan F. Pinter, and Jeannie T. Lee. PAR-TERRA directs homologous sex chromosome pairing. Nature Structural & Molecular Biology . 2017 July 10, doi:10.1038/nsmb.3432

3.Athanasios Zovoilis , Catherine Cifuentes-Rojas, Hsueh-Ping Chu , Alfredo J. Hernandez, and Jeannie T. Lee. Destabilization of B2 RNA by EZH2 activates the stress response. Cell . 2016 Dec 15 Volume 167, Issue 7, p1788–1802.e13

4.Yi Liao, Hsueh-Ping Chu * , Zhixain Hu, Jason J. Merkin, Jianmin Chen, Zuguo Liu, Kurt Degenhardt, Eileen White and Alexey G. Ryazanov. Paradoxical roles of elongation factor-2 kinase in stem cell survival . * CO-FIRST AUTHOR . J Biol. Chem. 2016 July 27 ( jbc.M116.724856 )

5.Hsueh-Ping Chu , Yi Liao, James S. Novak, Zhixian Hu, Jason J. Merkin, Yuriy Shymkiv, Bart P. Braeckman, Maxim V. Dorovkov, Alexandra Nguyen, Peter M, Clifford, Robert G. Nagele, David E. Harrison, Ronald E. Ellis, Alexey G. Ryazanov. Germline quality control: eEF2K stands guard to eliminate defective oocytes. Developmental Cell . 2014 Mar 10;28(5):561-72.

6.Martin A. Bewley, Trong K. Pham, Helen M. Marriott, Josselin Noirel, Hsueh-Ping Chu , Saw Y. Ow, Alexey, G. Ryazanov, Robert C.Read, Moira K. B. Whyte, Benny Chain, Phillip C. Wright and David H. Dockrell. Proteomic evaluation and validation of cathepsin D regulated proteins in macrophages exposed to Streptococus pneumonia . Molecular & Cellular Proteomics . 2011 Jun;10 (6)M111.008193

7.Grace S. Shieh, Tsai-Hung Fan, Hsueh-Ping Chu (2008). A Bayesian approach to assessing differential expression of microarray data. Journal of Statistical Computation and Simulation , Volume 78, Issue 2 February 2008, p.179 – 191

8.Hsu-Shui Wu, Hui-Ting Lin, Chi-Kuang Leo Wang, Yen-Feng Chiang, Hsueh-Ping Chu , Meng-Chun Hu (2007) . Human CYP11A1 promoter drives Cre recombinase expression in the brain in addition to adrenals and gonads. Genesis . 2007 Feb;45(2):59-65.

9.Noomen Ben El Hadj, Meng-Chun Hu, Hsueh-Ping Chu , Leo Chi-Kuang Wang, and Bon-chu Chung (2006)- Phenotypic Analysis of Mice with Steroid Deficiency. Genetically Engineered Mice Handbook , Taylor&Francis. Edited by John P. Sundberg and Tsutomu Ichiki. Chapter 19, p253- 262, 2006.

10.Martin Heine, Catharina I. Cramm-Behrens, Athar Ansari, Hsueh-Ping Chu , Alexey G Ryazanov, Hassan Y Naim, and Ralf Jacob. Alpha-kinase 1, a new component in apical protein transport. J Biol. Chem . 2005 Jul 8;280(27):25637-43

11.Meng-Chun Hu, Nai-Chi Hsu, Noomen en El Hadj, Chin-I Pai, Hsueh-Ping Chu , Chi-Kuang Leo Wang, and Bon-Chu Chung, Steriod Deficiency Syndromes in Mice with Targeted Disruption of Cyp11A1. Mol Endocrinol , 2002.16(8)p.1943-1950.





MCB5036 非編碼RNA 專題討論
MCB7002 專題研究
MCB5007 分子細胞生物學
MCB8001 博士班專題討論
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